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dr mike heilemann  (Addgene inc)


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  • 93

    Structured Review

    Addgene inc dr mike heilemann
    Dr Mike Heilemann, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dr+mike+heilemann/pirespuro2-CTLA4-mEos2+(Plasmid+%2398285)/pmc12748717-231-22-25
    Average 93 stars, based on 3 article reviews
    dr mike heilemann - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Elevated intracellular copper induces CTR1 monomerization and prevents copper uptake
    Article Snippet: The Cu-transfer deficient mutant construct, CTR1(M150L) mEos , was further generated by site-directed mutagenesis (Oligo #4 and #5, Supplementary Table ) using Q5 site-directed mutagenesis kit (New England Biolab E0554S) following the manufacturer’s instructions. .. The plasmid expressing CTLA4 mEos was constructed by replacing the mEos2 fluorescent protein in the original plasmid pirespuro2-CTLA4-mEos2, a kind gift from Dr. Mike Heilemann (Addgene plasmid # 98285; RRID:Addgene_98285) , with PCR-amplified mEos4b-Flag fragment (oligo #6 and #7, Supplementary Table ) between the AgeI and NotI restriction sites for experimental consistency with our other constructs. ..

    Expressing:

    Article Title: Elevated intracellular copper induces CTR1 monomerization and prevents copper uptake
    Article Snippet: The Cu-transfer deficient mutant construct, CTR1(M150L) mEos , was further generated by site-directed mutagenesis (Oligo #4 and #5, Supplementary Table ) using Q5 site-directed mutagenesis kit (New England Biolab E0554S) following the manufacturer’s instructions. .. The plasmid expressing CTLA4 mEos was constructed by replacing the mEos2 fluorescent protein in the original plasmid pirespuro2-CTLA4-mEos2, a kind gift from Dr. Mike Heilemann (Addgene plasmid # 98285; RRID:Addgene_98285) , with PCR-amplified mEos4b-Flag fragment (oligo #6 and #7, Supplementary Table ) between the AgeI and NotI restriction sites for experimental consistency with our other constructs. ..

    Construct:

    Article Title: Elevated intracellular copper induces CTR1 monomerization and prevents copper uptake
    Article Snippet: The Cu-transfer deficient mutant construct, CTR1(M150L) mEos , was further generated by site-directed mutagenesis (Oligo #4 and #5, Supplementary Table ) using Q5 site-directed mutagenesis kit (New England Biolab E0554S) following the manufacturer’s instructions. .. The plasmid expressing CTLA4 mEos was constructed by replacing the mEos2 fluorescent protein in the original plasmid pirespuro2-CTLA4-mEos2, a kind gift from Dr. Mike Heilemann (Addgene plasmid # 98285; RRID:Addgene_98285) , with PCR-amplified mEos4b-Flag fragment (oligo #6 and #7, Supplementary Table ) between the AgeI and NotI restriction sites for experimental consistency with our other constructs. ..

    Polymerase Chain Reaction:

    Article Title: Elevated intracellular copper induces CTR1 monomerization and prevents copper uptake
    Article Snippet: The Cu-transfer deficient mutant construct, CTR1(M150L) mEos , was further generated by site-directed mutagenesis (Oligo #4 and #5, Supplementary Table ) using Q5 site-directed mutagenesis kit (New England Biolab E0554S) following the manufacturer’s instructions. .. The plasmid expressing CTLA4 mEos was constructed by replacing the mEos2 fluorescent protein in the original plasmid pirespuro2-CTLA4-mEos2, a kind gift from Dr. Mike Heilemann (Addgene plasmid # 98285; RRID:Addgene_98285) , with PCR-amplified mEos4b-Flag fragment (oligo #6 and #7, Supplementary Table ) between the AgeI and NotI restriction sites for experimental consistency with our other constructs. ..



    Similar Products

    93
    Addgene inc dr mike heilemann
    Dr Mike Heilemann, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dr+mike+heilemann/pirespuro2-CTLA4-mEos2+(Plasmid+%2398285)/pmc12748717-231-22-25
    Average 93 stars, based on 1 article reviews
    dr mike heilemann - by Bioz Stars, 2026-10
    93/100 stars
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